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Fig. 5 Knockdown of Bmp signal rescues Engrailed expression in sly pioneers and MFFs and ectopically activates Engrailed expression in wild-type slow and fast lineages. (A,E,I,M) Lateral views of wild-type (A), sly (E), dorsomorphin-treated sly (I) and dorsomorphin-treated wild-type embryos (M) at 26 hpf. Anterior is leftwards, dorsal is upwards. (B-D,F-H,J-L,N-P) Magnification views (projection of confocal sections) of the regions around somite 10 (B-C,F-G,J-K,N-O) and somite 18 (D,H,L,P) for each type of embryo. (A-P) 4D9 (in green) labels pioneer (arrowheads) and MFF nuclei; Prox1 (in red) labels slow muscle fibre nuclei, including pioneers. Because of the projection, some nuclei artificially overlap. All pioneer nuclei are therefore identified by arrowheads. (A,C,G,I,K,O) 4D9 labelling only. The asterisk locates ectopic tails in dorsomorphin-treated embryos. Scale bar: 50 μm.