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Fig. 5 Lack of Tie-2 protects zebrafish embryos from cranial hemorrhages caused by treatment with statins. (A) Graphic representation (in percentage) of hemorrhagic (orange bars) and non-hemorrhagic (yellow bars) groups for wild-type siblings (i) and tie-2 mutants (ii) after exposure to increasing concentrations of atorvastatin; the numbers of hemorrhagic (orange bars) and non-hemorrhagic (yellow bars) groups for wild-type siblings (iii) and tie-2 mutants (iv) after exposure to increasing concentrations of simvastatin are shown. (B) tie-2 mutants show similar cranial hemorrhages (arrow) as the wild-type embryos after treatment with atorvastatin. (C) No cranial hemorrhages are observed in wild-type siblings and tie-2 mutants as shown by o-dianizidine staining. However, treatment with 1000 nM atorvastatin causes cranial hemorrhages in the head region of both genotypic groups (asterisks show the regions in the head area of siblings and mutants where blood is accumulating).