ZFIN Image: Lee et al., 2010, Fig. 4

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Figure Caption

Fig. 4

Expression of the Light Chain of Tetanus Toxin in Dorsal Habenula Neurons Prevents Avoidance Learning

(A) Kaede expression in habenula neurons (arrows) of an 18-day-old GAL4^s1019t/UAS:Kaede fish. This image is a projection of optical sections spanning 90 μm.

(B) Expression of Kaede (red) relative to Ron [37] (cyan), a protein found in the dorsal habenula. This image is a projection spanning 18 μm.

(C) A 3-week-old triple transgenic (GAL4^s1019t/UAS:Kaede/UAS:TeTXlc-CFP). Habenula neurons expressing TeTXlc-CFP (green) are in the medial regions. There is incomplete overlap with Kaede (red) expression.

(D and E) Avoidance response (D) and mean swim speed (E) in the probe trial following conditioning with paired CS and US for fish carrying GAL4^s1019t/UAS:Kaede/UAS:TeTXlc-CFP, GAL4^s1019t/UAS:Kaede, or UAS:TeTXlc-CFP.

(F) Avoidance response as a function of trial number.

Fish are shown in dorsal view; anterior to the left. Scale bars represents 50 μm. s1019t refers to the GAL4^s1019t line. The dotted line in (C) is the midline. Error bars indicate SEM. ^**p < 0.001; ^*p < 0.05.

Acknowledgments

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