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Fig. 7 Localization and translational control of in vitro-transcribed mRNA in oocytes. (A) Whole-mount gfp in situ hybridization of a full-grown oocyte fixed 4 h after injection with tgo3′ mRNA. (B) Dextran fluorescence of the full-grown oocyte 4 h after injection. The in vitro-transcribed tgo3′ mRNA and dextran appeared to be diffused after injection. (C) The times when translation of the injected mRNA occurs after MIH stimulation. Translational repression of the tgo3′ mRNA was derepressed before (TGVBD = 0) or after MIH treatment, with timings much earlier than that of the mRNA transcribed in vivo ( Fig. 5G).
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Reprinted from Developmental Biology, 348(1), Yasuda, K., Kotani, T., Ota, R., and Yamashita, M., Transgenic zebrafish reveals novel mechanisms of translational control of cyclin B1 mRNA in oocytes, 76-86, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.