Fig. 6

Fig. 6 Cytoplasmic polyadenylation of cyclin B1 and reporter mRNAs during oocyte maturation. (A) Cytoplasmic polyadenylation of cyclin B1 (cyclin B1), tgo3′ (tgo3′) and tg3′ (tg3′) mRNAs in immature (Im) and mature (M) oocytes. The poly(A) tails of all mRNAs remained short in immature oocytes and were elongated in mature oocytes. The efficiency of polyadenylation of zebrafish cyclin B1 is lower than that of Xenopus cyclin B1 because of the differences in the 3′ UTR sequences. Bars indicate the elongated poly(A) tail. (B) Time courses of polyadenylation of cyclin B1, tgo3′ and tg3′ mRNAs. Dotted lines indicate the basal size of the poly(A) tails. Asterisks indicate when polyadenylation of each mRNA is initiated. Polyadenylation of cyclin B1 and tgo3′ mRNAs was initiated at the time T_GVBD50 = 60, while that of tg3′ mRNA was initiated at T_GVBD50 = 25. (C) The times that polyadenylation of cyclin B1 and reporter mRNAs occurs after MIH stimulation. Error bars indicate mean ± s.e.m., n = 3, asterisk, P < 0.05 (Student′s t-test).