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Fig. 3

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ZDB-IMAGE-101124-4
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Figures for Yin et al., 2010
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Fig. 3 Hand2 Is Required for Gut Looping and LPM Migration

(A) foxa3 expression in wild-type and hanc99 mutants. Upper panel: the gut has looped to the left by 35 hpf in wild-types (22/22), but stays in the midline in hanc99 mutants (17/18). Dashed lines mark the midline. Lower panel: in wild-type by 48 hpf, the liver and pancreas are positioned on the left and right side of the gut, respectively (35/35). In hanc99 mutants, the liver (arrows) and/or pancreas (asterisks) is duplicated (12/12).

(B) At the 20-somite stage (19 hpf), spaw is expressed in the left LPM (arrows) in wild-types (70/70), but is absent from the LPM in most hanc99 mutants, or is bilateral in a few hanc99 mutants.

(C–G) Time course analysis shows that the Tg(hand2:EGFP)-expressing cells in hanc99 mutants fail to undergo asymmetric rearrangements. Ten hanc99 mutants were analyzed at each time point. Dashed lines outline the LPM.

(H) Diagram of LPM migration in hanc99 mutants. The gut is in yellow, the left LPM is in red, the right LPM is in blue, and the Tg(hand2:EGFP)-expressing cells are in green. The dark red line represents the apical side of the LPM epithelium. Arrows indicate the direction of migration of the Tg(hand2:EGFP)-expressing cells.

(A and B) Dorsal views, anterior to the top.

(C–H) Transverse sections, dorsal to the top.

L, left; Li, liver; NC, notochord; Pa, pancreas; R, right; So, somite. The scale bars represent 50 μm. See also Figure S2.

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Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell