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Fig. 2 Stereotypical Germ-Layer Progenitor Cell Division Orientation Is Not Required for Body-Axis Elongation during Gastrulation
(A–D) Body-axis elongation (A) and ntl and hgg1 expression in wild-type (B), emi1 (C), and wild-type embryos treated with cell-division inhibitors (D) at bud stage (10 hpf).
(E–H) Body-axis elongation (E) and ntl and hgg1 in wild-type (F), MZfz7a/b embryos treated with DMSO (G) and MZfz7a/b embryos treated with cell-division inhibitors (H) at bud stage (10 hpf).
(I–K) Frequency distribution of pairs of division-orientation angles (θ,φ) in DynIC-Ab-injected embryos during gastrulation (6–10 hpf). Distribution of azimuthal (θ) (J) and elevation (φ) angles (K). Chi-square test on the combined angles: wild-type versus DynIC-Ab (p < 0.005).
(L–N) Body-axis elongation (L) and ntl and hgg1 expression in wild-type (M) and DynIC-injected embryos (N) at bud stage (10 hpf).
Error bars show the standard deviation.
See also Figure S3 and Table S1.