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Fig. S1 Schematic diagram of zebrafish metastasis model under normoxia and hypoxia. Mammalian tumor cells were labeled with DiI dye in vitro and approximately 300 tumor cells in 5 nL DMEM are injected into the perivitelline space of each 48 h post-fertilization Tg(fli-1:EGFP) transgenic zebrafish embryo. Tumor cell invasion, dissemination, metastasis and angiogenesis were monitored in vivo in living fish body at different time points under normoxia and hypoxia (7.5% air saturation-the maximal tolerated oxygen tension) using fluorescent microscopy.
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Full text @ Proc. Natl. Acad. Sci. USA