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Fig. S5 gata6 expression level are not modulated by miR-145 at early embryonic stages. (A and B) gata6 expression levels (white arrowhead) are unchanged in myocardial precursors of uninjected wild-type embryos and miR-145 morphants at 16–19 hpf. (C and D) gata6 expression is significantly increased in miR-145 morphants at 48 hpf. (Scale bars, 200 μm.) (E) There is no significant change in gata6 expression in miR-145 morphants at 16 hpf and 24 hpf by qPCR.
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