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Fig. S6 Strong phenotype triggered by lidocaine treatment. (A) Control conditions (B) After treatment with lidocaine, 17% (n = 36) embryos do not have endothelial tissue thickening. (C and D) bmp4 expression in (C) lidocaine-treated and (D) untreated embryos. In treated embryos, the heart tube is very immature, a situation very similar to that observed in the no-flow conditions reported in [14]. Such embryos were not used for flow analyses or qPCR, nor were they tested for valve morphogenesis at later stages. White arrow points to the AV canal.
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