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Fig. S3 Decreased blood cells number do not affects heart chamber patterning as well as head and trunk vasculogenesis. (A, B, F, G, K, and L) nppa and bmp4 expression is unaffected in gata1 (F and G) and gata2 (K and L) morphants compared to controls (A and B) showing that heart chambers and AV canal patterning is normal when blood cell numbers decrease. (C-E, H-J, and M-O) GFP expression in Tg(flk1:EGFP) delimitates the cardiovascular system as it is limited to every endothelial cells in the embryo (C, H, and M). Details of the head (D, I, and N) and trunk (E, J, and O) vasculature in controls (C–E), gata1 (H-J), and gata2 (M-O) show that no obvious malformation of the cardiovascular system is visible when blood cell number decreases. Arrows in (D, I, and N) point to the fourth branchial arch; arrows in (E, J, and O) point to secondary sprouts of the trunk cardiovascular wiring. Panels (C, H, and M) are each a composite of two original images.