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Fig. S1 Altered midbrain gene expression in morphant embryos. Lateral (A-F, J-L) and dorsal (G-I, M-O) views anterior to the left, or anterior views (P-R) dorsal to the top of embryos either un-injected (A, D, G, J, M, P) or injected with MO1a + MO1b (B, E, H, K, N, Q) or control mMO1a + mMO1b (C, F, I, L, O, R). All embryos are at 48 hpf, except for J-L, which are at 24 hpf. Arrowhead in B and E indicates the reduced expression in the dorsal midbrain and arrow in E indicates reduced expression in the hindbrain. Arrowhead in G, H and M, N demarcates the position of the MHB. Arrowhead in P, Q indicates the optic stalk region. MO, morpholino; mMO, mismatch morpholino; MHB, midbrain-hindbrain boundary; hpf, hours post-fertilization.