Fig. S13

Fig. S13 Expansion of Wnt activity is not reduced by knockdown of atoh1 in the mib1 pLLp. (A,B) Expression of lef1 in wild-type, mib1^m178, atoh1a and atoh1b knockdown (atoh1MOs) pLLp, and following atoh1a and atoh1b knockdown in mib1 mutants (mib1+atoh1MOs) at 31 hpf (A) and 35 hpf (B). lef1 expression is expanded both in atoh1MOs (n=10 at 31 hpf, n=11 at 35 hpf) and mib1^m178 (n=10 at 31 hpf, n=16 at 35 hpf) pLLp. Expanded lef1 expression in mib1^m178 pLLp is not reduced in mib1 ^m178+atoh1MOs (n=6 at 31 hpf, n=13 at 35 hpf). (C) A comparison of the size of the lef1 expression domains in wild-type, mib1^m178, atoh1MOs and mib1^m178+atoh1MOs. The numbers of embryos used in this study are as follows: wild-type embryos, n=11 at 31 hpf, n=11 at 35 hpf; atoh1MOs embryos, n=10 at 31 hpf, n=11 at 35 hpf; mib1^m178 mutant embryos, n=10 at 31 hpf, n=16 at 35 hp; mib1^m178+atoh1MOs embryos, n=6 at 31 hpf, n=13 at 35 hpf. Statistical significance was measured using the Student′s t-test. *P<0.0005.