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Fig. 2 Abnormal NMJs are observed in larval and adult G93R os10 fish. (A) Whole-mount antibody labeling with synaptic vesicle 2 (SV2, red) for pre-synaptic vesicles and α-bungarotoxin (BTX, green) for post-synaptic terminals from nTg (top) and G93R os10 (bottom) 11-dpf larvae. (B) Quantitation of NMJ properties in larval (11 dpf) G93R os10 zebrafish in comparison to nTg controls. (C) Adult NMJs were examined in trunk sections by immunolabeling with antibodies to SV2 and α-BTX. (D) Quantitation of NMJ properties in adult (12 month) G93R os10 zebrafish and nTg controls (*P<0.02, n=10 sections from three larvae or adult fish per genotype). Bars, 20 μm.