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Fig. 7 In vitro binding analysis of BBS8 and Vangl2. A) Co-immunoprecipitation of BBS8-myc with Vangl2-GFP using an anti-GFP antibody for the immunopercipitation and an anti-myc antibody for the Western blot. Empty-GFP, empty-myc and a random GFP construct were used as negative controls. BBS2-GFP, a known binding partner of BBS8 was used as a positive control. Positive co-IP bands are only observed with Vangl2-GFP and BBS8-myc and in the positive control. B) GST pull-down assay of BBS8 and Vangl2. myc-Vangl2 was pulled-down with BBS8-GST and concentrated, as compared to GST-only control.
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Reprinted from Developmental Biology, 345(2), May-Simera, H.L., Kai, M., Hernandez, V., Osborn, D.P., Tada, M., and Beales, P.L., Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish, 215-225, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.