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Fig. S8 Ve-cadherin is required for endothelial tubulogenesis in the ISVs. Confocal images of the ISVs from Tg(fli1:egfp)y1 transgenic recipient embryos at 54 hpf transplanted with cells from a double EGFP- and RFP-expressing Tg(fli1:egfp)y1/Tg(β-actin:rfp) donor embryo. Lateral images of living embryos, dorsal is up, anterior is to the left. (A-C) Normal endothelial tubulogenesis in a donor embryo injected with 10 ng of the control MO. (D-F) Endothelial tube formation was inhibited when the donor embryo was injected with 10 ng of the ve-cadherin MO. Scale bars: 10 μm.
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