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Fig. S4 In vitro kinase assay with or without BI 2536. High concentration of BI2536 affects the activities of Plk2 and Plk3 as well as Plk1 in vitro. Bacterially expressed zebrafish Plk1, Plk2 and Plk3 were subjected to in vitro kinase assay. 100μM of BI 2536 efficiently inactivated the kinase activity of all three zebrafish Plks in vitro.
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Reprinted from Developmental Biology, 345(1), Jeong, K., Jeong, J.Y., Lee, H.O., Choi, E., and Lee, H., Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos, 34-48, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.