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Fig. 4 Human and zebrafish parkin co-aggregate.
HeLa cells grown on glass coverslips were transiently transfected with FLAG-tagged zebrafish parkin and FLAG-tagged human parkin. 24 h after transfection the cells were exposed to hydrogen peroxide (10 mM) for 30 min. The cells were then fixed and analyzed by indirect immunofluorescence using an anti-HA polyclonal and an anti-FLAG monoclonal antibody. Nuclei were stained by DAPI (blue).
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