Vacuolated cells selectively express GFP in the 214A-GFP transgenic fish line. Control MO or jag1a/1b MOs were injected into 214A-GFP at the one-cell stage. Images were taken from transverse sections of embryos at 32 hpf. Arrows indicate the nuclei of GFP-positive cells (vacuolated cells), and arrowheads indicate the nuclei of GFP-negative cells (non-vacuolated cells). Note that the number of vacuolated cells increased in the jag1a/1b knockdown embryos (jag1a1bMOs).
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