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Fig. 5 The ErbB inhibitors PD168393 and AG1478 phenocopy the mutant phenotypes observed in kiz and hps. (A and B) Compared to Mock incubated controls the number of neuromasts in the lateral line system doubles (C) and the regenerative response is blocked (D) upon incubation with either 10 μM PD168393 or AG1478. (E) The function of ErbB receptors is needed before 36 hpf in the lateral line system and its inhibition blocks growth post-injury during the regeneration response in the caudal fin even when added at 48 hpi (F). The data is expressed as Incubation time in the inhibitor (h)/time when inhibitor was added (hpi). As the inhibitor irreversibly binds to ErbB the data corresponds to 24h/48, 48h/24 hpi and 72h/0hpi (F). RR length (see Fig. 1D) was digitally measured at 8 dpf/3 dpi using the Scaling option in AxioVision 4.4. Anterior to the left, dorsal up.
Reprinted from Developmental Biology, 327(1), Rojas-Muñoz, A., Rajadhyksha, S., Gilmour, D., van Bebber, F., Antos, C., Rodríguez Esteban, C., Nüsslein-Volhard, C., and Izpisúa Belmonte, J.C., ErbB2 and ErbB3 regulate amputation-induced proliferation and migration during vertebrate regeneration, 177-190, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.