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Fig. 1 SCORE tools. (A) Anesthetized larval zebrafish are placed into a methylcellulose (pictured) or low-melting-point agarose solution before being loaded into the capillary housing. (B) A pipette pump with capillary adaptor is used to facilitate loading of zebrafish into the capillary housing. (C) Multiple larvae (arrows) can be imaged in a single capillary, allowing for rapid screening of mutants. (D) A corrective solution (water pictured) is placed upon the capillary to reduce distortion. Foam barriers, marked by arrows, are placed upon the glass slide to help contain corrective solution and to provide a friction surface for ease of rotation.