Fig. 4

Fig. 4 CyA mediated attenuation of Hh signaling is enhanced by Rest knockdown. Lateral views of 26 hpf control (A, C, E, G) and rest morphants (B, D, F, H) embryos stained for nkx2.2a (A–D) or pax3a (E–H). Embryos were incubated in control media (A, B, E, F) or 1.5 μM cyclopamine (CyA) media from 6 hpf on (C, D, G, H). rest morphants incubated in control media show a modest increase in nkx2.2a expression (B, compare to control, A) and a modest decrease in pax3a (F, compare to control, E) grown under the same conditions. 1.5 μM CyA decreases nkx2.2a expression (C) and modestly expands pax3a (G) in control embryos. Rest knockdown produces a greater reduction in nkx2.2a expression, and a greater expansion of pax3a, then CyA treatment alone.