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Fig. S7 Hedgehog signalling is required before 15S for correct anteroposterior patterning of the zebrafish otic vesicle. (A-F) Merged confocal z-stacks of maculae stained with FITC-phalloidin to reveal the actin-rich stereocilia of the sensory hair cells at 84 hpf. Lateral views: anterior to left, dorsal to top. 50 μM cyclopamine was applied to wild-type (A,B) embryos from the time shown in the grey box until 24 hpf. When cyclopamine was applied from 8-9S, the resulting otic vesicles resembled those of smo-/- (A, 3/6 embryos) or con-/- (B, 3/6 embryos) embryos, which are both severe Hh loss-of-function mutants (Hammond et al., 2003). When cyclopamine was applied from later stages (C-F), the Hh loss-of-function otic phenotypes were not fully phenocopied. Numbers in parentheses in the grey boxes show the proportion of embryos displaying the phenotype shown. Scale bar: 50 μm. am, anterior (utricular) macula; pm, posterior (saccular) macula.