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Figure Caption
Fig. S5 ptc2 expression is raised in ptc1-/- and ptc1-/-; lep-/- homozygotes. In situ hybridisation for ptc2. Dorsal views of whole-mount 26 hpf embryos, anterior to left, showing the two otic vesicles and midline tissue expressing ptc2. (A,B) Levels of ptc2 expression are slightly raised in ptc1-/- homozygotes compared with sibling embryos. (C,D) Levels of ptc2 are increased considerably in ptc1-/-; lep-/- double homozygotes compared with siblings. The genotype of ptc1-/- embryos was confirmed by PCR. Scale bar: 50 μm.
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