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Fig. 5 Expression of AP markers is altered in ptc1-/-; lep-/- otic vesicles. (A-L) Expression of anterior (hmx3, fgf8, pax5), ventral/central (otx1) and posterior (fst1) otic markers. In ptc1-/-; lep-/-, expression of anterior markers is reduced or absent, otx1 expression is reduced, and posterior fst1 expression (arrow, K) is lost in the ear but upregulated in surrounding tissues. (A-H) Lateral views, anterior to left, dorsal to top. (I-L) Dorsal views; anterior to left, lateral to bottom. Scale bars: 50 μm (in left-hand image of each pair).
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