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Fig. S2

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ZDB-IMAGE-100408-11
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Figures for Veldman et al., 2010
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Figure Caption

Fig. S2 Tuba1a-targeting MOs do not stimulate RGC apoptosis. tuba1a-targeting MO was delivered to RGCs, in vivo, by placing MO-soaked Gelfoam on the sectioned optic nerve stump. The next day the Gelfoam was removed and 3 days later retinas were isolated, sectioned and assayed for cell death using TUNEL stain. Note that like the uninjured retina, MO-treated retinas showed little cell death. Positive control is a nuclease-treated retina. Brown signal indicates cells undergoing apoptosis.

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Reprinted from Molecular and cellular neurosciences, 43(4), Veldman, M.B., Bemben, M.A., and Goldman, D., Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish, 370-383, Copyright (2010) with permission from Elsevier. Full text @ Mol. Cell Neurosci.