IMAGE

Fig. 2

ID
ZDB-IMAGE-100401-12
Genes
Source
Figures for Lewis et al., 2001
Image
Figure Caption

Fig. 2 The less Hh signal, the fewer primary motoneurons, but all embryos still form median floor plate. Lateral views of islet2 expression in blue (A,B,D,E) and α-collagen type II expression in red (A,B) at 18-21 h (18-22 somites) in wild-type uninjected embryo (A); MO-injected syu mutant (B); and embryos from a triple mutant cross (D,E). (B) entirely lacked primary motoneurons but still formed median floor plate. (D) has no islet2-expressing primary motoneurons and is presumably a cyc-/-;flh-/-;syu-/- triple mutant. (E) has only six islet2-expressing primary motoneurons but has a notochord (*) and is probably cyc-/-;flh+/-;syu-/- or cyc-/-;flh+/+;syu-/-. (C) shows total number of embryos from all the morpholino injection experiments that formed six or fewer islet2-expressing primary motoneurons. These are broken down into embryos from syu+/- parents (blue) or syu+/-;smu+/- parents (pink) injected with twhh-MO + ehh-MO and the one syu mutant injected with twhh-MO that formed six primary motoneurons (green). (F) The number of motoneurons in individual triple mutants. These were embryos from cyc+/-;flh+/-;syu+/- parents that had no notochord and five or fewer primary motoneurons. islet2 experiment, n=609: expect about 9.5 triple mutants; observed eight embryos with five or fewer motoneurons. islet1 experiment, n=549: expect about 8.5 triple mutants; observed seven embryos with five or fewer motoneurons. islet1 + islet2 experiment, n=367: expect about 5.7 triple mutants; observed four embryos with four or fewer motoneurons. Scale bar: 100 μm (A,B) and 250 μm (D,E).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development