ZFIN Image: Li et al., 2010, Fig. 6

Image

Figure Caption

Fig. 6 Mutating the motifs AATGAA, AATGGA, GAGCGG, and GAGGGG impairs the forebrain enhancer activity of CNE1.01. (A) Alignment of zebrafish and human CNE 1.01. Abbreviations: D, distal to the transcription start site; P, proximal to the transcription start site. (B) Functional validation of de novo predicted motifs in the CNE 1.01. Reporter activity of (1) The CNE containing wildtype copies of the predicted motifs, or with the following motifs mutated: (2) the distal copy of the motif AATGAA; (3) the proximial copy of the motif AATGAA; (4) the motif GAGCGG; (5) the motif AATGAA; (6) and (7) the motif GAGGGG.

Acknowledgments

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Reprinted from Developmental Biology, 337(2), Li, Q., Ritter, D., Yang, N., Dong, Z., Li, H., Chuang, J.H., and Guo, S., A systematic approach to identify functional motifs within vertebrate developmental enhancers, 484-495, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.