ZFIN Image: Wright et al., 2009, Fig. 5

Image

Figure Caption

Fig. 5 Nrarp expression is Notch-dependent in fish, chick, and mouse, but Wnt-dependent only in mouse. A-D: Hamburger and Hamilton (HH) stage 11-12 chick (A,C) and embryonic day (E) 9.5 (B,D) half embryo culture analysis. Both sides were cultured with or without (A,B) 100 μM DAPT to inhibit the Notch activity or (C,D) 200 μM CKI-7 to inhibit the Wnt activity. Both half explants were hybridized with an Nrarp probe. E-I: nrarp-a expression analyzed in the PSM of 10-somite stage zebrafish embryos from (E) wild-type (F) aei/deltaD mutant, and (G) des/notch1a mutant. Zebrafish embryos stained for nrarp-a expression after being incubated in the (H) absence or (I) presence of 200 μM CKI-7 to inhibit Wnt activity. Rostral to the top.

Acknowledgments

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