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Fig. 5 Ectopic mouse Wnt4 expression induces a non-canonical Wnt phenotype during zebrafish development. (A) Gain of function phenotypes induced by mouse Wnt4 in zebrafish embryo. Various doses of mouse Wnt4 or Wnt1 mRNA was injected to fertilized zebrafish eggs, and the phenotype was assessed 24 hrs after injection. (β-galactosidase (lacZ) mRNA was used as a negative control.) The phenotypes were classified into 3 groups (severe, strong and mild) based on the complexity of phenotype. (B) Wnt4 induces gain of function phenotypes in a dose dependent manner. On the y-axis, % total embryos are shown with each (color-coded) phenotype (severe to mild, shown in A), after injection with the indicated dose of control, Wnt1 or Wnt4 constructs.