ZFIN Image: Takeda et al., 2009, Fig. S2

Image

Figure Caption

Fig. S2 DAZL counteracts the miRNA repression. (A) Schematic representation of the 3′UTR of GFP-IPT^miR-204 tdrd7 ΔmiR-430 site mRNA. The target site of miR-204 (blue) and the mutated site for miR-430 (gray) are shown. (B) Sequences of wildtype and mutated miR-430 target sites in the tdrd7 3′UTR. Nucleotide positions relative to the stop codon were shown above. Nucleotides that basepair with miR-430 seed are indicated in blue. (C) The GFP-IPT^miR-204 tdrd7 ΔmiR-430 site and DsRed mRNAs were injected with or without the mRNA encoding Myc-DAZL at the one-cell stage. Subsequently, the miR-204 duplex was injected at the two-cell stage. GFP and DsRed were analyzed at 24 hpf. Fold change of normalized GFP fluorescence relative to mock control is shown on the right.

Acknowledgments

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