Fig. 3

Fig. 3 DAZL relieves the miR-430-mediated repression via binding to the cis-element GUUC.

(A) Schematic representation of the 3′UTR of GFP-3xIPT^miR-430 6x#16 and GFP-3xIPT^miR-430 6xmt2. Sequences of IPT^miR-430 and #16 are shown below. The sequence that basepairs with miR-430 seed (blue) and the DAZL-binding sequence (red) are indicated. (B) Injection experiments of GFP-3xIPT^miR-430 6x#16 (a and b) or GFP-3xIPT^miR-430 6xmt2 (c and d) with or without the mRNA encoding Myc-DAZL. DsRed mRNA was co-injected. GFP and DsRed expression were analyzed at 24 hpf. Fold change of normalized GFP fluorescence relative to mock control is shown on the right. (C) In situ hybridization of the embryos injected with GFP-3xIPT^miR-430 6x#16 or GFP-3xIPT^miR-430 6xmt2 with a GFP probe at 24 hpf. The boxed region in the schematic representation was enlarged. Arrowhead indicates the PGCs.