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Fig. 6 Analysis of detached fibres indicates that muscle attachment failure occurs external to the sarcolemma. Whole-mount immunohistochemistry of mutant embryos at 72 hpf reveals the presence of dystrophin and β-dystroglycan at the ends of detached fibres. (A-F) Wild-type (A-C) and sof (D-F) embryos stained with anti-dystrophin. (G-L) Wild-type (G-I) and sof (J-L) embryos stained with anti-β-dystroglycan. (A,D,G,J) DIC images, (B,E,H,K) fluorescence images, (C,F,I,L) merged images. Arrows indicate fibre detachments. All panels show lateral views with anterior to the left. dys, anti-dystrophin; bDG, anti-β-dystroglycan.