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Fig. S4 Neural and somite patterning in iguana mutants. (A-H) Control embryos (igu/+ or +/+) (a,c,e,g) and igu/igu mutants (b,d,f,h) at 24 hpf were stained for the expression of ptc1 (A,B), nkx2.2b (c,d), olig2 (e,f) and eng2 (g,h). igu/igu mutants show dampened, but expanded, ptc1 expression (B). Accordingly, nkx2.2b expression in the neural tube is lost in igu/igu mutants (D), whereas olig2 expression in the neural tube (F) and eng2 expression in the somite (H) are expanded. Lateral views. Scale bar: 100 μm.
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