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Fig. S3 MZovl mutants have randomized left-right patterning. (a-e) MZovl mutants (b-e) display randomized lft2 expression compared with wild-type embryos (a). Symmetric eng2 expression (asterisks) in the midbrain-hindbrain boundary remains normal in MZovl mutants and is used as an internal landmark to compare lft2 expression between embryos. Examples of MZovl mutants with lft2 expression on the left (b), right (d), or both sides (c), or with no lft2 expression (e), are shown. Dorsal views of 22-somite stage embryos. Scale bar: 50 μm. (f) Quantification of left-right defects in MZovl mutants. Of wild-type embryos, 96% (n=100) show lft2 expression on the left side, whereas MZovl mutants (n=45) show randomized lft2 expression: 24% on the left side (B), 27% on the right side (D), 40% bilaterally (C), and 9% with no expression (E).