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Fig. 4 The Cardiac Actin-RFP cassette is effective as an internal control of transgenesis. The Cardiac Actin-RFP cassette drives expression of RFP in the somites. A: The broad RFP expression in the muscles of this embryo indicates high efficiency of integration. B: In this embryo, the transgenesis procedure was less effective. Thus, the possibility of germ line integrations is reduced.
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