Fig. S3

Fig. S3 The confirmation of the specific activities of six1a-MO, myod-MO and myf5-MO in zebrafish embryos. Co-injection of six1a-MO (8 ng), myod-MO (4 ng) or myod-MO (4 ng) with its counterpart mRNA (100 pg) was employed into one-celled stage of zebrafish embryos. The Six1a-GFP fusion protein was detected at 24 hpf in embryos injected with a six1a-egfp mRNA (A). The Six1a-GFP fusion protein was not detected at 24 hpf in embryos co-injected with six1a-egfp mRNA and six1a-MO (E). The wobble Six1a-GFP fusion protein was detected at 24 hpf in embryos injected with a wobble six1a-egfp mRNA (B). The Six1a-GFP fusion protein was detected at 24 hpf in embryos co-injected with a wobble six1a-egfp mRNA and six1a-MO (F). The Myod-GFP fusion protein was detected at 24 hpf in embryos injected with myod-egfp mRNA (C). The Myod-GFP fusion protein was not detected in embryos co-injected with myod-egfp mRNA and myod-MO (G). Te GFP was detected in embryos injected with myf5-MO-targt-egfp mRNA at 24 hpf (D). The GFP signal was absent in embryos co-injected with myf5-MO-targt-egfp mRNA and myf5-MO (H). Embryos injected with egfp mRNA alone (I), co-injected with egfp mRNA and six1a-MO (J), egfp mRNA and myod-MO (K) and egfp mRNA and myf5-MO (L) served as control groups.