ZFIN Image: Coffin et al., 2009, Fig. 8

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Figure Caption

Fig. 8 Interplay of amiloride and calcium in aminoglycoside uptake and hair cell death. (A) 50 μM amiloride reduces GTTR uptake under low calcium (300 μM) conditions (left) but does not completely prevent GTTR uptake even under higher (1500 μM) calcium conditions (right). Hair cell nuclei are labeled with YO-PRO-1 (blue), scale bars are 5 μm. (B) Amiloride increases hair cell survival proportionally regardless of calcium concentration. Black bars represent the effect of calcium only on hair cell loss for two concentrations of calcium (300 or 1500 μM). Dark gray bars depict hair cell survival in the indicated calcium concentration with 50 μM amiloride added, while light gray bars represent hair cell survival with 500 μM amiloride. Error bars are ±1 SD.

Acknowledgments

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Reprinted from Hearing Research, 253(1-2), Coffin, A.B., Owens, K.N., Raible, D.W., and Rubel, E.W., Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line, 42-51, Copyright (2009) with permission from Elsevier. Full text @ Hear. Res.