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Fig. S3 Zebrafish Pak1 Protein and Rescue. (A) Sequence alignment of Human and Zebrafish Pak1 protein. (B) Pak1 knockdown with a Pak1 MO to the 5′ intron/exon splice site (MO2) showed phenotypes identical to the Pak1 MO for the initial ATG codon. Co-injection of human Pak1 mRNA was able to recover the phenotype. Pictures were taken at a 12.5x magnification. (C) Quantification of zebrafish survival at 4 dpf in embryos injected with Pak1 MO2 and embryos rescued with human Pak1 wt-mRNA.
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
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