IMAGE

Fig. S1

ID
ZDB-IMAGE-090527-25
Source
Figures for Phelps et al., 2009
Image
Figure Caption

Fig. S1 KRAS Promotes Intestinal Cell Proliferation and Activation of β- Catenin following Loss of apc
(A) apcmcr zebrafish embryos were injected at the one cell stage with KRAS mRNA. The embryos were fixed at 72 hpf, sectioned and stained using hematoxylin and eosin (H&E). Shown are representative sagital sections. (B) Zebrafish embryos harboring an integrated β-catenin TOPdGFP reporter were injected with KRASG12D mRNA, apc morpholino or both. The embryos were fixed at 72 hpf and subjected to whole mount in situ hybridization using GFP as a probe. (C) mRNA was harvested from 72hpf embryos injected as in (B). PCR was performed for GAPDH and unspliced apc. All images represent at least three independent experiments. (Scale Bar: 10μm).

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell