Fig. 3

Fig. 3 Defects in circulatory function in kdrl mutants. (A–G) Transmitted light images of wild type and kdrl mutant zebrafish embryos at 55 h post fertilization. Lateral views, anterior to the left, dorsal is up. (A) Wild type sibling embryo. Scale bar is 250 μM. (B) kdrl^um6 mutant embryo displaying cardiac edema (white arrow). (C) Eye (indicated) and forebrain region of wild type sibling embryo. Scale bar is 50 μM. (D) Cranial hemorrhage (white arrows) in a heterozygous carrier of the kdrl^um6 allele. (E) kdrl^um6 heterozygous embryo. (F) kdrl^um19 mutant embryo. (G) Eye and forebrain region of a kdrl^um19 mutant embryo. A cranial hemorrhage is indicated by the white arrow. (H) Quantification of circulatory defects in clutches of embryos derived from incrosses of um6, um19, and y17 heterozygous carriers; circ — circulation, shunt — abnormal circulatory connection between dorsal aorta and posterior cardinal vein, hem — cranial hemorrhage.