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Figure Caption
Fig. 6 seahorse mutants retain the ability to interact with Dsh. Upper panel: western blot with anti-HA on samples pulled down by immunoprecipiatation with anti-Flag. Middle and lower panels are western blots of lysates with the indicated antibody to visualize the input of Sea proteins (HA) or Dsh (Flag). Note that both seatg238a and seafa20r can produce stable proteins in vivo. Both mutant Sea proteins can interact with Dsh, although the weaker interaction seen with Seafa20r may indicate that the truncation in this protein affects the binding site for Dsh.
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