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Fig. 1 Morphology of Ptx-injected embryos. (A, C, E) uninjected control, (B, D, F) Ptx-injected. (A, B) 30 hpf, lateral view; the injected embryo displays an altered shape of the head, blocky somites, and a generally disorganized tail. (C, D) 72 hpf, ventral view on head; the injected embryo displays cyclopia. (E, F) 96 hpf, lateral view on somites in trunk at level of anus; the somites of the injected embryo lack the chevron shape and the horizontal myoseptum (indicated in E by arrows). hpf, hours after fertilization.
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Reprinted from Developmental Biology, 194, Hammerschmidt, M. and McMahon, A.P., The effect of pertussis toxin on zebrafish development: a possible role for inhibitory G-proteins in hedgehog signaling, 166-171, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.