IMAGE

Fig. S2

ID
ZDB-IMAGE-090304-10
Source
Figures for Ellett et al., 2009
Image
Figure Caption

Fig. S2 Reporter assay validating the functional binding of ergMO to its target sequence. (A) Shows EGFP fluorescence in EGFP-only (control) and Target:EGFP (test) groups in the absence of erg ATG-targeting morpholino. (B) erg ATG-targeting morpholino efficiently knocks down EGFP expression in Target:EGFP but not EGFP-only injected embryos. Uninjected controls are shown for each experiment and wavelength. Rhodamine dextran dye was used a tracer for morpholino injection. Panels show pools of randomly selected embryos. Methodological details are in text. Top panels: bright field (BF); central panels: Rhodamine fluorescence; lower panels: EGFP fluorescence. Bright-field and fluorescent images were taken with 8 ms and 8 s exposure times, respectively.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 126(3-4), Ellett, F., Kile, B.T., and Lieschke, G.J., The role of the ETS factor erg in zebrafish vasculogenesis, 220-229, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.