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Fig. S4

ID
ZDB-IMAGE-090220-59
Source
Figures for Carreira-Barbosa et al., 2009
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Figure Caption

Fig. S4 Celsr-ΔC causes C&E and epiboly defects, and injection of a low dose of stbmMO, dvl2MO or Lyn-Celsr causes C&E but not epiboly defects in MZord embryos. MZord embryos (A,D) or MZord embryos injected with 0.4 pmoles stbmMO (B,E), 0.75 pmoles dvl2MO (C,F) or 20 pg Lyn-Celsr RNA (I,L). Wild-type embryos were injected with 300 pg Celsr-ΔC-HA RNA (H,K) or left uninjected (G,J). The embryos were fixed at tail-bud and subjected for in situ hybridisation with hgg1 for the prechordal plate, ntl for the notochord and dlx3 for the anterior edge of the neural plate. The results of C&E phenotypes are summerised in Table S1. Note that injection of 300 pg Celsr-ΔC-HA RNA causes C&E defects with posteriorly misplaced prechordal plate as well as epiboly defects shown by the shifted germ ring expression of ntl (indicated by an arrowhead).

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