IMAGE

Fig. S2

ID
ZDB-IMAGE-090127-48
Source
Figures for Kizil et al., 2009
Image
Figure Caption

Fig. S2 Morpholino-mediated knock-down of smp is specific and can be rescued. (A) Diagram of the genomic region encompassing exons 3 and 4 as well as intron 3 of smp. Symbols mark positions of the antisense splice-junction morpholino (splice mo) and PCR primers (In-R and In-L). Underlying panel is a gel picture with DNA ladder and RT-PCR of mismatch-morpholino-transfected (mm-mo) and antisense-splice-junction-morpholino-transfected (splice mo) embryo extracts. (B) 28 hpf embryos after injection with mm-mo. (C) Embryos at the same stage after injection with splice-mo. (D) Rescued phenotype of same-staged embryos after co-injection of smp mRNA with splice-mo. Numbers indicate embryos showing wild-type phenotype/total injected embryos. The percents are the ratios of observed wild-type phenotype to total. Embryos in (B–D) show emissions from fluorescein-tagged morpholinos. Scale bars equal 500 μm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 325(2), Kizil, C., Otto, G.W., Geisler, R., Nüsslein-Volhard, C., and Antos, C.L., Simplet controls cell proliferation and gene transcription during zebrafish caudal fin regeneration, 329-340, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.