Fig. 3

Fig. 3 Maternal and zygotic depletion of gene activity affects markers of dorsal–ventral patterning. (A–C) Animal pole view with dorsal to the right. (A) The dorsal extent of eve1 expression (arrowheads) was unaffected in embryos (70% epiboly) derived from ogo^m60/+ intercrosses or in (B) 75% of embryos (n = 84) from a cross between ogo^m60/+ males and ogo^m60/ogo^tm305 females. (C) The remaining 25% (presumed ogo^m60/ogo^m60) had dorsally expanded eve1 expression. (D–F) Dorsal views. (D) The extent of tbx6 expression (arrowheads) was unaffected in embryos (80% epiboly) from ogo^m60/+ intercrosses or in (E) 71% of the progeny (n = 34) of ogo^m60/ogo^tm305 females crossed to ogo^m60/+ males. (F) A dorsalward expansion of tbx6 expression was observed in the remaining 29%. (G–J) Posterior views. Compared to (G, I) their WT siblings, expression of (H) tbx6 (tailbud stage) and (J) eve1 (2 somite stage) was expanded in ogo^m60 mutant tailbuds. Scale bars, 200 μm.