ZFIN Image: Miller-Bertoglio et al., 1999, Fig. 6

Image

Figure Caption

Fig. 6 Mutant phenotypes are suppressed by injected chordin RNA. (A–C) Progeny of ogo^tm305/ogo^tm305 intercrosses injected with (A) GFP RNA or (B and C) a mixture of chordin and GFP RNA. Chordin-injected embryos were (B) partially or (C) fully rescued. (D–G) Progeny of ogo^m60/+ intercrosses injected with (D and E) GFP RNA or (F and G) a mixture of chordin and GFP RNA. (F) Partial rescue class A. (G) Partial rescue class B. Insets (D–G) show PCR analyses of the closely linked polymorphic marker (Z6924; left lane WT reference) used to confirm the genotype (right lane) of the depicted embryo. The PCR product was 200 bp for WT (EK) and 220 bp for ogo (HK). Indicated are the cell accumulation (arrowhead), the duplicated fin (arrow), and the vesicle (open arrow) characteristic of the mutant ventral tail. Scale bar, 250 μm.

Acknowledgments

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Reprinted from Developmental Biology, 214(1), Miller-Bertoglio, V., Carmany-Rampey, A., Fürthauer, M., Gonzalez, E.M., Thisse, C., Thisse, B., Halpern, M.E., and Solnica-Krezel, L., Maternal and zygotic activity of the zebrafish ogon locus antagonizes BMP signaling, 72-86, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.