ZFIN Image: Jiang et al., 2008, Fig. S5

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Figure Caption

Fig. S5 Exdpf is Specifically Required for Exocrine Pancreas Formation but not Gut or Liver

(A–C) Expression of GFP MP760GFP transgenic fish. (A) A control embryo injected with standard control morpholino. Note strong GFP expression in the pancreatic area at 24 hpf (arrow) and 2 dpf (arrowhead). (B) An example of embryo injected with exdpf mRNA (100 pg). Note strong GFP expression in the pancreatic area at 24 hpf (arrow) and 2 dpf (arrowhead). GFP expression in the developing gut is comparable to that in control embryo. (C) An example of embryo injected with exdpf morpholino (2ng of MO1^exdpf). Note no strong GFP expression in the presumed pancreatic area at 24 hpf (arrow). A weak GFP positive pancreatic bud can be observed at 2 dpf (arrowhead). GFP expression in the gut is still comparable to that in the control and exdpf RNA injected embryos.

(D–G) In situ hybridization using a ceruloplasmin probe with 3 dpf embryos. (D) A control embryo injected with standard morpholino control. (E–G) Examples of embryos injected with exdpf morpholino. Note comparable expression of cerulaplasmin in all embryos.

Acknowledgments

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