ZFIN Image: Berndt et al., 2008, Fig. 6

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Figure Caption

Fig. 6 Inhibition of myosin II or ROCK disrupts blebbing and causes defects in NCC EMT. (A–J) Time-lapse sequences of cells expressing GPI-GFP (membranes, blue) and RFP-UtrCH (F-actin, red) in vehicle treated (DMSO) (A–E) or 50 μM blebbistatin-treated (F–J) embryos. Arrowheads in A, C indicate normal blebs. Arrowhead in F indicates bleb that fails to retract after blebbistatin treatment. Dorsal view adjacent to rhombomere 2, anterior is right. Time is in seconds. (K–N) In situ hybridization for dlx2 in embryos treated with DMSO (K), or 10 μM (L) or 50 μM (M) blebbistatin, or 50 μM ROCK inhibitor (N). Dorsal views, anterior left. (O) Number of cells undergoing EMT per hour during 2 h movies. Blebbistatin (BBS) and ROCKi were at 50 μM. Scale bar = 10 μm for A–J and 40 μm for K–N.

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Acknowledgments

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Reprinted from Developmental Biology, 324(2), Berndt, J.D., Clay, M.R., Langenberg, T., and Halloran, M.C., Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo, 236-244, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.